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| Product Name | Coenzyme Ⅰ NAD (H) Content Assay Kit, 120T/25S |
| Catalog No. | EATK-YJL-0033 |
| Testing Equipment | Visible spectrophotometer |
| Number of Detectable Samples | 25 Samples |
| Testing Accessories | 1 mL Glass cuvette (d=10 mm) |
| Estimated Measurement Time | 4 h (25 Samples) |
| Storage | Store according to label instructions |
| Reagents to be Prepared | Anhydrous ethanol |
| Product Introduction | Nicotinamide adenine dinucleotide (NAD+/NADH) is a central coenzyme in cellular metabolism, functioning as a crucial redox carrier in numerous catabolic and anabolic pathways. The balance between its oxidized (NAD+) and reduced (NADH) forms reflects the cellular redox state and metabolic activity. This assay kit utilizes a sensitive enzymatic cycling method that amplifies the signal, allowing for the precise measurement of very low concentrations of total NAD(H), NAD+, and NADH in biological samples such as tissues, cells, and serum. |
| Product Application | • Measurement of cellular redox state and metabolic flux. • Research on mitochondrial function, aging, and sirtuin biology. • Studies of metabolic disorders, cancer metabolism, and drug mechanisms. • Evaluation of cellular energy status in response to stress or nutrients. |
| Product Advantages | • High Sensitivity & Specificity: Employs an enzymatic cycling reaction that generates a strong, measurable colorimetric signal from minute amounts of NAD+/H, providing exceptional sensitivity and specificity. • Differential Measurement: Includes separate protocols for quantifying total NAD(H), NAD+, and NADH, enabling the calculation of the critical NAD+/NADH ratio, a key metabolic indicator. • Stable, Lyophilized Reagents: Key enzymes and substrates are provided in stable, lyophilized form, ensuring long shelf life and consistent performance upon reconstitution. • Broad Sample Compatibility: Optimized for use with a wide range of sample types, including animal tissues, cultured cells, bacteria, and plant extracts, after appropriate deproteinization. |
For research use only, not for clinical use.
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