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| Product Name | Chicken DHT (Dihydrotestosterone) ELISA Kit |
| Catalog No. | HTK-YJL-0100 |
| Synonyms | DHT ELISA Kit, Dihydrotestosterone ELISA Kit |
| Species | Chicken |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Range | 39.063-2500 pg/ml |
| Sensitivity | 23.438 pg/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with DHT, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Dihydrotestosterone (DHT), a potent androgen derived from testosterone via the action of 5α-reductase, plays a critical role in the physiological and reproductive biology of chickens (Gallus gallus). As a key hormonal mediator, DHT is involved in regulating multiple essential processes in poultry, making its accurate quantification vital for advancing avian research.
Physiological Significance in Chickens: DHT influences the development and maintenance of male secondary sexual characteristics in chickens, such as comb growth, feather patterning, and mating behaviors. It also contributes to the regulation of reproductive tract development, sperm production, and overall reproductive fitness—factors that are crucial for poultry breeding programs and reproductive physiology studies.
Research Relevance in Avian Science: In modern poultry research, understanding DHT dynamics is linked to several high-priority areas. For instance, studies investigating the impact of environmental stressors (e.g., temperature fluctuations, chemical exposures) on avian endocrine function rely on precise DHT measurements to assess hormonal disruptions. Additionally, research on poultry growth performance, immune system modulation, and metabolic health often incorporates DHT analysis, as emerging evidence suggests that DHT may interact with other hormonal pathways (e.g., estrogen signaling) to influence immune responses and nutrient metabolism in chickens.
Need for Specific Assay Tools: Traditional methods for hormone detection (such as radioimmunoassay, RIA) have limitations in terms of safety, cost, and specificity for avian samples. ELISA kits tailored for chicken DHT address these gaps by offering a non-radioactive, cost-effective, and species-specific solution. Unlike general-purpose hormone assays, chicken-specific DHT ELISA kits are optimized to recognize the unique molecular structure of chicken DHT, minimizing cross-reactivity with other androgens (e.g., testosterone, androstenedione) or avian-specific metabolites—ensuring reliable results for poultry-focused studies.
Application Scope Across Sample Types: The demand for flexible sample compatibility drives the utility of this kit. Chicken DHT research often requires analyzing diverse sample matrices: serum and plasma for systemic hormone levels, cell culture supernatants to study DHT secretion in vitro (e.g., from ovarian or testicular cells), and tissue lysates (e.g., from hypothalamus, testes, or adrenal glands) to investigate localized DHT production and tissue-specific hormonal regulation. This kit’s ability to handle all these sample types makes it a versatile tool for both in vivo and in vitro avian endocrine research.
Species-Specific Detection: The kit is exclusively optimized for chicken (Gallus gallus) DHT, ensuring high specificity that eliminates obvious cross-reactivity with other androgen analogues (e.g., testosterone, 5α-androstane-3α,17β-diol) or avian-specific hormones. This avoids false-positive or false-negative results common with non-species-specific assays, guaranteeing data accuracy for poultry research.
Broad Detection Range: With a detection range of 39.063–2500 pg/ml, the kit can quantify both low and high DHT concentrations in chicken samples. This flexibility accommodates diverse research scenarios, from measuring basal DHT levels in healthy chickens to detecting elevated or suppressed levels in experimentally treated groups (e.g., hormone-manipulated or stress-exposed birds).
High Sensitivity: Boasting a sensitivity of 23.438 pg/ml, the kit can detect even trace amounts of DHT in chicken samples. This is critical for studies focusing on early-stage hormonal changes (e.g., DHT fluctuations during chick development) or subtle differences in DHT levels between experimental groups—ensuring no meaningful biological signal is missed.
Rapid Assay Workflow: The total reaction duration is only 2 hours, significantly reducing experimental time compared to longer assays (e.g., 3-hour or overnight protocols). This allows researchers to process more samples in a single day, improving laboratory efficiency without compromising result quality.
Versatile Sample Compatibility: The kit supports multiple chicken sample types, including serum, plasma, cell culture supernatant, and cell/tissue lysate. This eliminates the need for separate assays for different sample matrices, simplifying experimental design and reducing overall research costs.
Stable Storage Conditions: The kit can be stored at 2–8°C (sealed, no cryopreservation required), making it easy to handle and store in standard laboratory refrigerators. This stability (with a defined shelf life) ensures consistent performance over time, reducing the risk of reagent degradation and assay failure.
Reliable Reproducibility: The kit’s design (based on competitive ELISA with pre-coated antigen) minimizes intra-assay and inter-assay variability. This ensures that results are consistent across multiple experiments and between different users, a key requirement for publishing high-quality research and validating experimental findings.
User-Friendly Protocol: The assay does not require specialized technical skills or complex equipment beyond a standard microplate reader (set to 450 nm). Clear, step-by-step instructions guide users through sample preparation, incubation, and detection, making it accessible to both experienced researchers and laboratory technicians new to hormone ELISA assays.
Cost-Effective for Routine Use: Available in 48T and 96T formats, the kit allows laboratories to choose the size that matches their sample volume needs. This prevents waste from unused reagents and reduces per-sample testing costs, making it ideal for both small-scale pilot studies and large-scale research projects.
Research-Focused Design: The kit is developed specifically for research use, aligning with the needs of avian endocrinology, developmental biology, and poultry science studies. Its performance characteristics (e.g., specificity, sensitivity) are tailored to address the unique challenges of chicken DHT detection, ensuring it meets the rigorous standards of academic and industrial research.
Minimal Sample Requirement: The kit works with small sample volumes (consistent with standard ELISA practices for similar matrices), which is particularly valuable when working with limited or precious chicken samples (e.g., serum from young chicks or rare poultry breeds). This reduces the need for excessive sample collection, minimizing stress on experimental animals and complying with ethical research guidelines.
For research use only, not for clinical use.
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