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| Product Name | Antinuclear Antibody Spectrum Test Kit (Microarray Chip Method) |
| Catalog No. | MK-QCY-0011 |
| Test Method | Gene microarray method |
| Nucleic Acid Type | DNA |
| Species | Human |
| Application | Used to detect IgG antibodies in serum. |
| Storage | Store at -20°C and properly avoid repeated freezing and thawing. |
Antinuclear Antibody (ANA) testing is a critical tool in the field of autoimmune disease research, as ANAs are autoantibodies that target various nuclear components (such as nucleic acids, nuclear proteins, and ribonucleoproteins) and are closely associated with a range of systemic autoimmune rheumatic diseases (SARDs) and other autoimmune conditions. In research settings, the detection of specific ANA subtypes (e.g., anti-Sm, anti-SSA, anti-dsDNA) plays a key role in studying the pathogenesis, disease progression, and subtype classification of autoimmune disorders—including but not limited to systemic lupus erythematosus (SLE), Sjögren’s syndrome, scleroderma, and myositis.Traditional ANA detection methods, such as indirect immunofluorescence (IIF) or enzyme-linked immunosorbent assay (ELISA), often have limitations: IIF can only provide qualitative screening results without identifying specific antibody subtypes, while ELISA typically requires separate tests for each target, leading to low efficiency, high sample consumption, and prolonged experimental cycles. With the development of microarray technology, microarray chip-based ANA spectrum test kits have emerged as a advanced solution for research applications. These kits leverage the high-throughput nature of microarray platforms to detect multiple ANA subtypes simultaneously from a single sample, addressing the inefficiencies of traditional methods and meeting the growing demand for comprehensive, rapid ANA profiling in autoimmune disease research.This Antinuclear Antibody Spectrum Test Kit (Microarray Chip Method) is designed specifically for research use, focusing on the detection of IgG-class antibodies against nuclear antigens in human serum. It provides researchers with a reliable tool to explore the association between specific ANA subtypes and autoimmune disease phenotypes, supporting preclinical studies, biomarker discovery, and mechanism research in the field of immunology.
High-throughput parallel detection: Enables simultaneous analysis of multiple antinuclear antibody subtypes in a single experiment, eliminating the need for separate tests for each target and significantly reducing experimental time and labor.
Research-focused sample compatibility: Optimized for human serum samples, a common biological matrix in autoimmune disease research, ensuring compatibility with standard sample collection and processing workflows in research laboratories.
Specific detection of IgG antibodies: Targets IgG-class autoantibodies, which are the primary antibody isotype associated with chronic autoimmune responses and are widely studied in the context of autoimmune disease pathogenesis.
Stable storage conditions: Requires storage at -20°C with protection against repeated freezing and thawing, ensuring long-term stability of reagents and consistent performance across multiple research batches.
Microarray chip-based technology: Utilizes gene microarray principles to immobilize specific nuclear antigens on a chip surface, enabling visualized qualitative or semi-quantitative readouts (when paired with a microarray reader) for easy result interpretation in research settings.
Time and cost efficiency: By reducing the number of individual experiments needed to profile multiple ANA subtypes, the kit lowers overall reagent consumption and labor costs, making it suitable for large-scale research projects or high-volume sample analysis.
Reduced sample requirement: Designed to work with small volumes of serum (consistent with typical microarray platform needs, e.g., as low as 15μL in similar technologies), which is critical for research studies where sample availability is limited (e.g., patient-derived samples or rare disease cohorts).
Reliable performance: Aligns with the performance characteristics of advanced microarray-based ANA tests, including high sensitivity (detection limits comparable to or lower than traditional methods, e.g., below 3 IU/mL for key targets like dsDNA in similar research-grade kits) and good reproducibility (intra-assay and inter-assay coefficients of variation <15%), ensuring consistent results for research data validity.
Flexibility for research applications: Supports a wide range of research objectives, from initial ANA screening in cohort studies to detailed subtype analysis for biomarker validation, adapting to diverse research needs in immunology and autoimmune disease research.
Compatibility with standard research equipment: Works with common microarray readers (commercially available in research laboratories) for quantitative result analysis, integrating seamlessly into existing research workflows without the need for specialized, custom equipment.
For research use only, not for clinical use.
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