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| Product Name | Alkaline Phosphatase (ALP) Activity Assay Kit, 96 T (80 Samples), 48 T (32 Samples) |
| Catalog No. | CMAK-YJL-0082 |
| Size | 96 T (80 samples), 48 T (32 samples) |
| Sample Type | Serum (plasma); tissues; cells |
| Detection Instrument | Microplate reader (500-530 nm, optimal detection wavelength: 520 nm) |
| Research Field | Liver and kidney function |
| Sensitivity | 0.13 King unit/100 mL |
| Detection Range | 0.13-50 King unit/100 mL |
| Accuracy | Inter-assay variation: 8.5% | Intra-assay variation: 5.1% |
| Sample Size | 5 μL |
| Storage | The product can be stored at 2-8°C, protected from light for 6 months. |
| Shelf Life | 6 months |
| Index Abbreviation | ALP |
| Detection Time | 30 min |
| Intended Use | For research use only. |
Alkaline Phosphatase (ALP), also known as AKP, is a group of specific phosphomonoester hydrolases that play a crucial role in multiple physiological processes within the human body and various biological systems. It exhibits optimal activity under alkaline conditions, which is the key characteristic behind its name.
Widely distributed across essential tissues and organs, ALP can be found in the liver, bones, intestines, kidneys, and placenta of humans. In the liver, it is closely associated with liver function, as abnormal ALP levels often serve as an important indicator of liver disorders such as cholestatic damage and other liver diseases. In bone tissue, ALP is a key marker of bone metabolism and mineralization processes. It participates in skeletal mineralization by hydrolyzing extracellular diphosphate— a potent inhibitor of mineralization—into phosphate, thereby promoting the formation of hydroxyapatite crystals and increasing the concentration of inorganic phosphate, which is vital for bone formation and the maintenance of bone health.
Beyond its roles in liver and bone function, ALP also undertakes important biochemical functions in other biological processes. It can catalyze the removal of 5'-phosphate groups from nucleic acid molecules, converting the 5'-P ends of DNA or RNA fragments into 5'-OH ends. Additionally, it is capable of removing phosphate groups from molecules such as nucleotides, proteins, and alkaloids, performing dephosphorylation reactions that regulate the activity and function of these biomolecules.
In scientific research and clinical-related studies, the accurate detection of ALP activity is of great significance. It serves as a critical biomarker in the research fields of liver and kidney function, bone metabolism, and the diagnosis and study of related diseases. For instance, in the study of liver diseases, changes in ALP activity can provide insights into the extent of liver damage and the progression of the disease. In bone metabolism research, monitoring ALP activity helps in understanding processes like osteoblast differentiation and bone turnover, which is essential for studying bone-related disorders such as osteoporosis and bone fractures. Our Alkaline Phosphatase (ALP) Activity Assay Kit is specifically developed to meet the demand for precise, efficient, and reliable detection of ALP activity in various biological samples, supporting researchers in advancing their studies in these important research fields.
Broad Sample Compatibility: The kit is designed to handle multiple sample types commonly used in scientific research, including serum (plasma), tissues, and cells. This versatility eliminates the need for researchers to purchase different kits for different sample sources, simplifying experimental workflows and reducing overall research costs.
High Sensitivity for Low-Level Detection: With a sensitivity of 0.13 King unit/100 mL, the kit can accurately detect even low levels of ALP activity in samples. This feature is particularly valuable for studies where ALP concentrations are inherently low, ensuring that no subtle changes in enzyme activity are missed, which is crucial for obtaining accurate and meaningful research results.
Wide Detection Range for Versatile Applications: It offers a detection range of 0.13-50 King unit/100 mL, covering a broad spectrum of ALP activity levels. Whether researchers are working with samples containing low, moderate, or high ALP activity, this kit can provide reliable quantitative results, making it suitable for a wide range of research scenarios in the fields of liver and kidney function, as well as bone metabolism.
Rapid Detection for Time Efficiency: The entire detection process can be completed within 30 minutes. This short detection time significantly speeds up experimental progress, allowing researchers to process more samples in a shorter period, improve work efficiency, and accelerate the pace of their research projects.
Good Accuracy for Reliable Results: The kit demonstrates excellent accuracy, with an inter-assay variation of 8.5% and an intra-assay variation of 5.1%. Low variation ensures that the detection results are highly reproducible, whether the assay is performed in the same batch (intra-assay) or across different batches (inter-assay). This reliability is essential for ensuring the validity and credibility of research data.
Convenient Storage Conditions: The product can be stored at 2-8°C while being protected from light, with a shelf life of 6 months. This storage requirement is easy to meet in most laboratory settings, eliminating the need for specialized storage equipment (such as ultra-low temperature freezers) and reducing the risk of kit degradation due to improper storage. The 6-month shelf life also provides researchers with sufficient time to plan and complete their experiments without worrying about the kit expiring prematurely.
Specific Detection Wavelength for Precise Reading: The optimal detection wavelength is 520 nm (with a detection range of 500-530 nm), which is compatible with most standard microplate readers commonly available in laboratories. This compatibility ensures that researchers can easily use the kit with existing equipment, without the need for additional investments in specialized detection instruments.
Supports Accurate Research in Target Fields: Tailored for the research field of liver and kidney function, the kit’s performance parameters (such as sensitivity, detection range, and accuracy) are optimized to meet the specific needs of studies in this area. It enables researchers to obtain precise ALP activity data, which is essential for analyzing and understanding the physiological and pathological changes related to liver and kidney function, thereby providing reliable support for advancing research in these fields.
Minimizes Sample Consumption: Only 5 μL of sample is required per assay. This minimal sample volume is a significant advantage, especially when working with precious or limited samples (such as rare clinical specimens or samples obtained from small animal models). It allows researchers to maximize the use of available samples, perform more replicate experiments, and obtain more comprehensive data without wasting valuable sample resources.
Ensures Consistent Experimental Outcomes: The combination of low inter-assay and intra-assay variation ensures that the results obtained from the kit are highly consistent. This consistency is critical for research projects that require long-term data collection or multi-batch sample analysis, as it reduces the impact of experimental variability on research conclusions, making the data more comparable and credible.
Simplifies Experimental Operations: The kit’s design takes into account the practical needs of laboratory operations. With no complex pre-treatment steps required for most sample types and a straightforward detection protocol, it lowers the technical threshold for using the kit. Even researchers with less experience in enzyme activity detection can easily master the operation, reducing the risk of experimental errors caused by complicated procedures.
Cost-Effective for Research Projects: Available in two sizes (96 T for 80 samples and 48 T for 32 samples), the kit allows researchers to choose the appropriate size based on their experimental scale and sample quantity. This flexible sizing helps avoid unnecessary waste of kit reagents (when a large kit is used for a small number of samples) or the need for frequent kit purchases (when a small kit is insufficient for a large number of samples), thereby optimizing research costs.
For research use only, not for clinical use.
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