| Product Name | Human Erythrocyte RhD Genotyping Kit (PCR-SSP) |
| Catalog No. | BTRTK-HMM-0013 |
| Description | The main reasons for common difficult RhD blood groups include pathological interference and subtyping, etc. RhD blood group genotyping test can avoid the above interference, which is a necessary guarantee for safe blood transfusion. For RhD-negative pregnant women, mother/father can be tested prenatally, and if necessary, amniotic fluid can be tested to determine the fetal genotype and predict the risk of fetal hemolytic disease of the newborn. This kit can be used as a primary screening. |
| Applications | This kit can be used to detect genes, including RhD-positive genes and RhD-negative genes, of which RhD-negative genes include RhD exon total deletion type, weak D15 type (EXON6 845 G/A), RhD-CE(2-9)-D2 type, DEL RhD1227A pure type (EXON9 1227A/A). |
| Sample Type | Whole blood ≥1 mL (sodium citrate or EDTA anticoagulation); DNA ≥ 20 μL (concentration 20-40 ng/μL, A260/A280 value 1.6-2.0); Amniotic fluid: ≥ 4ml (more than 16 weeks), the above three specimens 2-8 ℃ preservation of 2 weeks, ≤ -20 ℃ preservation of 1 year. Oral mucosa swabs (2, respectively, with brushing force to collect oral mucosa cells from both cheeks, stored at room temperature for 1 week). |
| Detection Methods | PCR-SSP |
| Estimated Measurement Time | 1 hour and 30 minutes (one-step (10min) operation, amplification products do not need to be transferred for spiking, avoiding laboratory and aerosol contamination). |
| Shelf Life | 12 months |
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