| Product Name | Horseradish peroxidase colorimetric kit |
| Catalog No. | CA-0085 |
| Description | Horseradish peroxidase colorimetric kit is a kit for immunotissue color development with the help of horseradish peroxidase (HRP). |
| Application | This product is used for color development of horseradish peroxidase bound to cells or tissues during immunohistochemistry or in situ hybridization, and can also be used to detect endogenous horseradish peroxidase in cells or tissues. |
| Storage | Store at -20ºC. |
| Transportation Condition | Transportation with cold packs/dry ice. |
Also Known As: Horseradish Peroxidase Colorimetric Kit, also referred to as HRP Activity Assay Kit or Peroxidase Detection Kit.
Enzyme Overview: Horseradish Peroxidase (HRP) is a versatile enzyme commonly used in various biochemical and immunological assays due to its ability to catalyze the oxidation of substrates in the presence of hydrogen peroxide. This enzyme is widely used as an indicator for chemical reactions that produce peroxide and is often conjugated to antibodies for use in enzyme-based immunoassay systems.
Functions and Applications: HRP plays a crucial role in several biological processes, including the removal of hydrogen peroxide, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, and response to environmental stresses such as wounding, pathogen attack, and oxidative stress. These functions may vary depending on the specific isozyme or isoform present in different plant tissues.
Scientific Significance: The enzyme binds one heme B (iron-protoporphyrin IX) group and two calcium ions per subunit, and its monomeric subunits make it an ideal candidate for a wide range of applications in research and diagnostics.
·High Sensitivity and Specificity: The kit is designed to provide accurate and reliable results, ensuring high sensitivity and specificity for the detection of HRP activity.
·Colorimetric Detection: Utilizes a colorimetric method, allowing for easy and visual detection of HRP activity through a spectrophotometer.
·Non-Species Specific: Suitable for a wide range of samples, as it is non-species specific, making it a versatile tool for various research applications.
·Easy to Use: The kit is designed for simplicity and ease of use, with clear instructions and a straightforward protocol, making it accessible for both novice and experienced researchers.
·Time-Efficient: Delivers results in a relatively short assay time of approximately 60 minutes, enabling faster research and development processes.
·Cost-Effective: Provides an economical solution for the detection of HRP activity, making it an ideal choice for laboratories with varying budgets.
·Wide Application Scope: Suitable for use in a variety of settings, including research labs, diagnostic centers, and academic institutions, enhancing its versatility.
·Reliable Performance: Backed by rigorous testing and validation, ensuring consistent and reliable performance every time.
For in vitro diagnostic use.
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