| Product Name | Single cell 3' RNA sequencing kit |
| Catalog No. | GW-00199 |
| Description | Single cell 3' RNA sequencing kit is a tool for single cell RNA sequencing that helps researchers to analyze and sequence gene expression in individual cells. This technique is very useful for studying differences in gene expression in complex biological systems, cellular diversity and its changes in different physiological and pathological states, and is an important tool for revealing cellular heterogeneity. |
| Application | The products are used to reveal cellular heterogeneity in biological samples and tissues, to identify rare or novel cell types that are relevant to pathogenesis or serve as biomarkers, and to help researchers understand developmental pathways and trajectories. |
| Storage | Store at 4-30°C. |
| Transportation Condition | Transportation at room temperature. |
| Technology | Droplet dispensing technology |
Single cell RNA sequencing (scRNA-seq) has revolutionized the field of biological research by shifting the focus from bulk cell analysis—where gene expression signals are averaged out and critical cellular differences are masked—to the study of individual cells. This technological leap has become indispensable for unlocking the complexity of biological systems, as traditional bulk sequencing fails to capture the heterogeneity that defines tissues, organs, and disease states.
The 3' RNA sequencing approach, a key subset of scRNA-seq, focuses on capturing the 3' end of mRNA transcripts. This method is particularly valuable for several reasons:
It enables high-throughput gene expression profiling, allowing researchers to analyze hundreds to thousands of cells in a single experiment—far more efficient than full-length transcript sequencing for large-scale studies.
It reduces sequencing costs per cell while maintaining sufficient sensitivity to detect differences in gene expression, making it accessible for labs with varying budget constraints.
It aligns with the needs of modern research, where understanding cellular diversity is critical to advancing fields like oncology, neuroscience, developmental biology, and immunology.
In oncology, for example, 3' scRNA-seq helps identify rare cancer stem cells or therapy-resistant cell clones within a tumor—populations that would be invisible in bulk sequencing. These rare cells often drive tumor progression, metastasis, and treatment failure, so their detection is key to developing targeted therapies. In neuroscience, the technique reveals the vast diversity of neuron subtypes and glial cells in the brain, shedding light on how specific cell populations contribute to neurodegenerative diseases like Alzheimer’s or Parkinson’s. In developmental biology, it tracks the trajectory of individual cells as they differentiate from stem cells into specialized tissues, uncovering the molecular pathways that guide embryonic development.
As research demands for higher efficiency, broader sample compatibility, and more accessible workflows have grown, the Single Cell 3' RNA Sequencing Kit (Cat.No: GW-00199) from Alta Diagno Tech has been developed to meet these needs. Built on droplet dispensing technology—a proven method for reliable single-cell isolation and barcoding—the kit addresses the core challenges of scRNA-seq: ensuring accurate capture of individual cells, minimizing RNA degradation, and delivering consistent, reproducible data. It is designed to support both basic research and translational studies, enabling researchers to explore cellular heterogeneity, identify novel cell types, and map developmental pathways without the need for overly complex equipment or specialized expertise.
Droplet Dispensing Technology for Reliable Single-Cell Capture: The kit leverages droplet dispensing technology to isolate individual cells into discrete, uniform droplets. This ensures that each cell is processed independently, preventing cross-contamination between cells and ensuring accurate association of gene expression data with individual cells—critical for detecting rare cell types or subtle expression differences.
Broad Sample Compatibility for Versatile Research: While optimized for standard cell suspensions, the kit’s gentle workflow and robust reagent formulation support analysis of diverse sample types commonly used in research, including primary cells (e.g., immune cells from blood or tissue), cell lines, and dissociated fresh or frozen tissue. This versatility eliminates the need for multiple kits for different sample sources, simplifying experimental design.
Stable Storage and Room-Temperature Transportation: With a storage range of 4-30°C and room-temperature transportation capability, the kit offers exceptional convenience for labs worldwide. It eliminates the need for expensive cold-chain shipping (e.g., dry ice) and reduces the risk of reagent degradation during transit, ensuring that the kit arrives in optimal condition regardless of location.
In Vitro Diagnostic (IVD) Grade Quality: Manufactured to meet IVD standards, the kit undergoes rigorous quality control checks to ensure consistency in reagent performance, batch-to-batch reproducibility, and data reliability. This quality assurance is critical for research that may eventually transition to clinical or diagnostic applications, such as identifying disease biomarkers.
Streamlined Workflow for Accessibility: The kit is designed with a user-friendly workflow that minimizes manual steps and does not require specialized, high-cost equipment (e.g., complex microfluidic devices). This makes it accessible to labs with limited experience in scRNA-seq, allowing more researchers to leverage single-cell technology without extensive training.
Cost-Effective for Scalable Research: Compared to high-end scRNA-seq platforms that require proprietary instruments or expensive consumables, the GW-00199 kit offers a more affordable entry point into 3' scRNA-seq. Its droplet-based technology delivers reliable data without the upfront investment in specialized equipment, making it ideal for labs looking to scale their single-cell studies without increasing budget burdens.
High Sensitivity for Detecting Subtle Expression Differences: The kit’s reagent formulation—including optimized lysis buffers and reverse transcription enzymes—minimizes RNA degradation and maximizes capture of polyadenylated mRNA (the primary target of 3' scRNA-seq). This high sensitivity ensures that even low-abundance transcripts are detected, enabling researchers to identify subtle gene expression changes that may be critical for understanding cellular function or disease mechanisms.
Reproducible Results for Consistent Research Outcomes: Through strict quality control of reagents and standardized droplet dispensing protocols, the kit delivers consistent results across experiments and between different users. This reproducibility is essential for validating findings (e.g., confirming the presence of a rare cell type in multiple samples) and ensuring that data can be reliably compared across studies.
Time-Efficient Workflow to Accelerate Research: The kit’s simplified workflow reduces hands-on time compared to complex microfluidic-based kits. While exact timelines may vary by experiment size, the core steps (cell capture, RNA extraction, and library preparation) are streamlined to minimize delays, allowing researchers to process samples and generate sequencing-ready libraries more quickly—accelerating the path from sample to data.
Support for In-Depth Heterogeneity Analysis: By enabling accurate single-cell capture and gene expression profiling, the kit empowers researchers to explore cellular heterogeneity in detail. It can identify rare cell populations (e.g., <1% of total cells) that are critical for research areas like cancer or immunology, as well as track changes in cell diversity across different physiological or pathological states (e.g., healthy vs. diseased tissue).
For in vitro diagnostic use.
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