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| Product Name | qPCR Mix kit |
| Catalog No. | P-001 |
| Description | The qPCR mix kit is a versatile intercalating dye mixture for robust, sensitive and rapid qPCR. it utilizes state-of-the-art technology and contains antibody-modulated, hot-start Taq polymerase and intercalating dyes for real-time PCR amplification. Optimized buffer chemistry as well as PCR enhancers and stabilizers allow for fast, sensitive qPCR. |
| Application | The qPCR mix kit is for robust, sensitive and fast qPCR, i.e. real-time PCR amplification. |
| Storage | Store at -20ºC. |
| Transportation Condition | Transportation with cold packs/dry ice. |
Real-time Polymerase Chain Reaction (qPCR) has become an indispensable core technology in modern molecular biology research, widely applied in gene expression analysis, pathogen detection, genotyping, and copy number variation analysis. Its ability to quantify nucleic acid targets with high sensitivity, specificity, and reproducibility makes it a cornerstone tool for laboratories worldwide. However, the performance of qPCR experiments heavily relies on the quality of qPCR reagents—especially the master mix, which integrates key components such as DNA polymerase, nucleotides, buffers, and detection dyes. Suboptimal master mixes often lead to challenges like non-specific amplification, low detection sensitivity, poor reproducibility between replicates, and failure to amplify low-abundance targets, all of which compromise experimental accuracy and efficiency.
Against this backdrop, our qPCR Mix Kit (Cat.No: P-001) is developed to address the critical needs of reliable qPCR performance in research settings. Drawing on advances in molecular biology reagent technology, the kit is engineered to overcome common pain points in qPCR workflows:
The Challenge of Non-Specific Amplification: Traditional Taq polymerase exhibits baseline activity at room temperature, leading to primer-dimer formation or off-target amplification during reaction setup. Our kit incorporates antibody-modulated hot-start Taq polymerase, which remains inactive at low temperatures and only activates after heat denaturation (typically at 95°C), effectively suppressing non-specific reactions before the first amplification cycle.
Demand for High Sensitivity: In research scenarios such as detecting rare pathogens or quantifying low-expression genes, qPCR reagents need to detect targets at the single-digit copy level. Our optimized buffer system, combined with high-purity dNTPs and PCR enhancers, ensures efficient amplification of even trace amounts of template DNA, avoiding false-negative results caused by insufficient sensitivity.
Need for Rapid and Robust Workflows: Modern research laboratories often handle large sample volumes, requiring qPCR reagents to support fast cycling protocols without sacrificing performance. The stabilizers and enhancer components in our kit enable rapid extension at 72°C (compatible with extension rates of 2–4 kb/min) while maintaining amplification efficiency, reducing total experiment time and increasing sample throughput.
Consistency Across Experiments: Batch-to-batch variation in reagents can lead to inconsistent results across different experimental runs. Our qPCR Mix Kit undergoes strict quality control (QC) processes, including testing for amplification efficiency (≥95% for standard templates), specificity (single melting curve peak), and reproducibility (Ct value variation ≤0.5 between replicates), ensuring stable performance across batches.
Antibody-Modulated Hot-Start Technology: The kit uses state-of-the-art antibody-modulated hot-start Taq polymerase. This polymerase is tightly bound by specific antibodies at room temperature, completely inhibiting its enzymatic activity to prevent primer-dimer formation and off-target amplification during reaction preparation. It only fully activates after a short heat denaturation step (e.g., 95°C for 10 minutes), ensuring high specificity from the first amplification cycle.
Versatile Intercalating Dye Detection: Integrated with a high-performance intercalating dye, the kit enables real-time monitoring of amplification products without the need for probe design or labeling. The dye specifically binds to double-stranded DNA (dsDNA) and emits strong fluorescence upon binding, ensuring accurate quantification of target nucleic acids while simplifying experimental design and reducing costs.
Optimized Buffer and Enhancer System: The buffer is formulated with a balanced concentration of Mg²⁺, pH stabilizers, and PCR enhancers (such as betaine and DMSO derivatives). This system enhances the amplification efficiency of templates with high GC or AT content, eliminates secondary structure interference in template DNA, and ensures consistent amplification performance across different target sequences.
Excellent Sensitivity for Low-Abundance Targets: The combination of high-purity dNTPs (free of DNase/RNase contamination) and the optimized enzyme-buffer system allows the kit to detect targets as low as single-digit to tens of copy numbers. This makes it ideal for research applications requiring detection of rare templates, such as low-expression genes or trace pathogens.
Rapid Amplification Compatibility: The kit supports fast qPCR cycling protocols. Its Taq polymerase exhibits a high extension rate (2–3 kb/min at 72°C), enabling completion of 40 amplification cycles in as little as 40–50 minutes (depending on the thermal cycler). This significantly reduces experiment time and increases sample throughput for high-volume research projects.
Long-Term Stability and Easy Storage: When stored at -20°C, the kit maintains full performance for at least 24 months without repeated freeze-thaw cycles affecting reagent quality. For short-term use (up to 72 hours), it can be kept at 4°C without loss of activity, providing flexibility for experimental workflows and minimizing reagent waste.
Superior Specificity Reduces Experimental Errors: Compared to conventional non-hot-start qPCR mixes, the antibody-modulated hot-start technology in this kit drastically reduces non-specific amplification. This results in single, sharp melting curve peaks (no primer-dimer or off-target peaks) and eliminates false-positive signals, ensuring that quantification data accurately reflect the true abundance of the target sequence.
High Reproducibility Ensures Data Reliability: The kit undergoes rigorous batch-to-batch quality control, including testing of amplification efficiency (CV ≤5% across replicates) and Ct value consistency (variation ≤0.3 between technical replicates). This minimizes intra- and inter-experiment variability, making it suitable for experiments requiring high data reproducibility, such as gene expression profiling or drug response studies.
User-Friendly Ready-to-Use Format: The kit is provided as a pre-mixed 2× qPCR Mix (containing polymerase, dye, dNTPs, and buffer), requiring only the addition of template DNA, primers, and nuclease-free water to set up reactions. This reduces pipetting steps by 50% compared to preparing reagents from individual components, lowering the risk of contamination and human error while simplifying workflow for both novice and experienced researchers.
Broad Compatibility with Experimental Systems: The kit is compatible with all major real-time qPCR instruments (including those with or without ROX reference dye channels) and works with a wide range of template types, such as genomic DNA (gDNA), complementary DNA (cDNA), and viral DNA. It also supports both standard and fast cycling modes, adapting to different laboratory equipment and experimental design needs.
Cost-Effective for Routine Research: Despite its high performance, the kit is priced competitively for routine research use. It is available in multiple pack sizes (100 rxn, 500 rxn) to match the sample volume needs of different laboratories—avoiding over-purchasing and reducing per-reaction costs for long-term, high-throughput projects.
Robust Performance in Challenging Templates: For templates with complex secondary structures (e.g., high-GC-content genes) or inhibitory substances (e.g., residual proteins from sample extraction), the kit’s buffer enhancers effectively mitigate these challenges. It maintains amplification efficiency above 90% for targets with GC content ranging from 30% to 75%, eliminating the need for time-consuming optimization of Mg²⁺ concentration or annealing temperature.
For research use only, not for clinical use.
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